FIG 5.
Signaling and adaptation behaviors of Tsr-GIGGG and Tsr-AIAAA. (A) Hill fits of serine dose-response data for pRR53 derivatives in FRET reporter strains UU2567 (R− B−) (white symbols) and UU2700 (R+ B+) (black symbols). The curves for wild-type Tsr (broken lines) are reproduced from graph one of Fig. 2C for UU2700 (short dashes) and UU2567 (long dashes). Gray lines indicate dose-response curves for the corresponding all-A and all-G variants of Tsr in UU2700, taken from graphs two and three of Fig. 2C, respectively. K1/2 and Hill coefficient values in UU2567 and UU2700 for these particular experiments were as follows: Tsr-AIAAA, 49 μM and 11 (white triangles) and 1.8 μM and 2.1 (black triangles); Tsr-GIGGG, 17 μM and 7.5 (white inverted triangles) and 3.8 μM and 3.3 (black inverted triangles). (B) Raw data (YFP/CFP) from FRET kinase assays with strain UU2700 (R+ B+) carrying the indicated pRR53 derivatives. Black triangles mark the time of addition of 3 μM serine; white triangles mark the time of serine removal. See the legends to Fig. 3B and 4A for additional details. The GIGGG response data were corrected for baseline drift, as described in Materials and Methods.