TABLE 3.

Cofactor specificities of purified AdhE proteins

Source of AdhE	Description	ALDH activitya		ADH activity
		NADH	NADPH	NADH	NADPH
C. thermocellum LL1004	Wild type	18.02 ± 2.45b	2.03 ± 0.46	42.23 ± 3.13	1.96 ± 1.25
C. thermocellum LL346	Ethanol tolerant	13.50 ± 3.54	0.43 ± 0.42	4.02 ± 1.48	0.03 ± 0.34
C. thermocellum LL350	Moderate ethanol production	31.50 ± 5.80	5.76 ± 0.16	42.67 ±7.46	42.30 ± 4.28
T. saccharolyticum LL1025	Wild type	10.63 ± 1.15	4.46 ± 1.63	17.43 ± 2.45	2.43 ± 2.43
T. saccharolyticum LL1049	High ethanol production	1.63 ± 0.30	11.13 ± 1.35	0.66 ± 1.97	12.70 ± 2.18
T. saccharolyticum LL1040	High ethanol production	0.07 ± 0.55	5.03 ± 2.05	0.01 ± 1.34	12.96 ± 4.54

^aActivity is expressed in units per milligram of protein (see Materials and Methods).

^bError represents 1 standard deviation (n = 4 to 8).