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. 2015 Jul 6;197(15):2499–2507. doi: 10.1128/JB.00094-15

FIG 2.

FIG 2

RcsB binds to the minC promoter region. Shown is binding of purified His-tagged RcsB to the minC upstream region. (A) Binding of the minC upstream region (290 bp) with increasing concentrations of RcsB. Lane 1 (from left), free labeled minC probe; lane 2, minC probe with 40 pmol RcsB; lane 3, minC probe with 75 pmol RcsB; lane 4, minC probe with 170 pmol RcsB; lane 5, minC probe with 340 pmol RcsB. (B) The digoxigenin-labeled 290-bp fragment was incubated with 340 pmol His-tagged RcsB. The binding was competed with unlabeled minC DNA (290 bp) and unlabeled abaI DNA (249 bp). Lane 1 (from left), free minC probe without RcsB or competitor; lane 2, minC probe with 340 pmol RcsB; lane 3, minC probe with 340 pmol RcsB and unlabeled minC DNA; lane 4, minC probe with 340 pmol RcsB and unlabeled abaI DNA.