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Semiquantitative RT-PCR of minC, flhD, and 16S RNA expression during swarmer cell differentiation. Total RNA was harvested at hourly intervals (T1, T2, T4, and T6) after placing cells on agar plates. The T0 sample represents the cells immediately before being placed on agar surfaces. PCRs on cDNA samples prepared from RNA at each time point were amplified for 10 cycles (16S rRNA), 30 cycles (minC), or 25 cycles (flhD).
