FIG 5.

A minC mutation reduces P. mirabilis swarming in wild-type and rcsB::Str^r backgrounds. (A) The minC::Kan^r mutation was introduced into PM7002 (wild type) in order to create PMKH1 (PM7002 minC::Kan^r). The complementing strain was created by introducing pMinCDE (pKNG101 plus minCDE) into PMKH1. (B) The minC::Kan^r mutation was also introduced into PMKM1 (PM7002 rcsB::Str^r) in order to create PMKH4 (PM7002 minC::Kan^r rcsB::Str^r). All 5 strains were grown overnight in LB broth containing the appropriate antibiotics and then equilibrated to the same optical density. To investigate their abilities to swarm, 4-μl droplets of the equilibrated inocula were spotted onto the same plate, and the migration distance was measured every 30 min. The averages of four measurements are shown for PM7002, PMKH1, PMKM1, PMKH4, and PMKH5. The error bars indicate standard deviations.