FIG 6.

The minC::Kan^r mutation causes an atypical swarm front. (A) Stationary-phase cultures of PM7002 (wild type), PMKH1 (PM7002 minC::Kan^r), and PMKH5 (PM7002 minC::Kan^r plus pMinCDE) grown overnight in LB broth were equilibrated to the same optical density and then spotted onto 1.5% LB agar contained in a Coverwell imaging chamber. Once the cells began to swarm, phase-contrast images of PM7002 (left), PMKH1 (center), and PMKH5 (right) were taken of the swarm front at ×1,000 magnification. (B) Western blot analysis of flagellin (FlaA) expression in PM7002 wild-type (lane 1), PM7002 minC::Kan^r (lane 2), and PM7002 minC::Kan^r plus pMinCDE (lane 3) cells harvested after 5 h growth on LB agar plates. (C) DAPI staining of chromosomes in PM7002 (wild-type) and PMKH1 (PM7002 minC::Kan^r) swarmer cells.