TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description and/or genotypea | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Laboratory stock |
| CC118 λpir | araD139 Δ(ara leu)7697 ΔlacZ74 phoAΔ20 galE galK thi rpsE rpoB argE(Am) recA1 | 37 |
| SM10 λpir | thi thr leu tonA supE recA RP4-2Tc::Mu Kanr λpir | 38 |
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) | Stratagene |
| P. mirabilis | ||
| PM7002 | Wild type; Tcr | ATCC |
| HI4320 | Wild type; Tcr | 39 |
| PMKH1 | PM7002 minC::Kanr | This study |
| PMKM1 | PM7002 rcsB::Strr | 10 |
| PMKH4 | PM7002 minC::Kanr rcsB::Strr | This study |
| PMKH5 | PM7002 minC::Kanr(pMinCDE) | This study |
| PMKH6 | PM7002(pMinClacZ) | This study |
| PMKH7 | PM7002 rcsB::Strr(pMinClacZ) | This study |
| PMKM2 | HI4320 rcsB::Strr | This study |
| Plasmids | ||
| pBluescriptII SK(−) | ColE1 replicon; lacZα Ampr | Stratagene |
| pBC | High copy number; Cmr | Stratagene |
| pKNG101 | R6K replicon; mob+ sacB+R+ Strr | 40 |
| pTrc99a | ColE1 replicon; trcPO lacIq Ampr | 41 |
| pQF50 | pRO1600 replicon; lacZα (promoterless); Ampr | 29 |
| pET21a | T7 expression vector; Ampr | Novagen |
| pRcsB | pKNG101::rcsB (internal fragment); Strr | 10 |
| pMinC | pKNG101 minC::Kanr Strr | This study |
| pBCMinCDE | pBC minCDE Cmr | This study |
| pMinCDE | pKNG101 minCDE Strr | This study |
| pMinClacZ | pQF50 minCp Ampr | This study |
| pETRcsB | pET21a rcsB Ampr | This study |
a
Kanr, kanamycin resistant; Tcr, tetracycline resistant; Strr, streptomycin resistant; Ampr, ampicillin resistant; Cmr, chloramphenicol resistant.