TABLE 2.
Primer sequences used in this study
| Primer | Sequence (5′→3′) | Purpose |
|---|---|---|
| minC-F | TTTTTCTAGATTTTTGAGCGAGGCCGGTAG | minC mutation |
| minC-R | TTTTGGATCCAGCGACTTGCCTGAATATTTTAGT | minC mutation |
| PminC-F | TTTTGTCGACGCGCGAGATCTAGCAACATT | minC promoter activity |
| PminC-R | TTTTGGATCCCCTGAGCTGCTGGCTTAGTT | minC promoter activity |
| GSP1 | CCAATATTGACCAGCAATGGAAACT | 5′-RACE |
| GSP2 | ACACCATAAATATGTACATTACCGTCT | 5′-RACE |
| minCSS-F | TTAAGAATTCCGCGAGATCTAGCAACATTGCA | DNA binding studies |
| minCSS2-F | TTAAGAATTCAGCGACTTGCCTGAATATTTTAGT | DNA binding studies |
| minCSS2-R | TTAAGAATTCAATGGGCGTGTTTGACATCT | DNA binding studies |
| abaI-F | AAGTTAATCGCTTCTTGCAG | DNA binding studies |
| abaI-R | AGCTTATGTGGTCGCTCAAG | DNA binding studies |
| minCDE-F | ATAATTAAAACACAGAGCC | Complementing strain |
| minCDE-R | CCAGATTATAGAAAATTTAC | Complementing strain |
| minCqRT-F | CCCAAGCGCCTCAATTTCTG | qRT-PCR studies |
| minCqRT-R | TTCCCACAACACGTAAGCCA | qRT-PCR studies |
| minDqRT-F | CCACCAACCCAGAAGTCTCC | qRT-PCR studies |
| minDqRT-R | CGGCCTGGATTATAGCGTGT | qRT-PCR studies |
| minEqRT-F | CGCGTAAAAAGTCGACAGCA | qRT-PCR studies |
| minEqRT-R | TAAGCTGGCTCAGTATCGCC | qRT-PCR studies |
| 16S-F | GGCTCAGATTGAACGCTGGC | qRT-PCR studies |
| 16S-R | CGAAGAGCCCCTGGTTTGG | qRT-PCR studies |
| flhD-F | TTGGTTAAGTTGGCTGAAAC | Semiquantitative RT-PCR |
| flhD-R | TTTGTAGCAGAGGTATCATG | Semiquantitative RT-PCR |
| minE-R | CTTAGGTGATTCTTCATTTTCAGG | Operon study |
| rcsB-F | ACAGTCTAGAGATCCGACAGTTGCTGTAGC | RcsB protein purification |
| rcsB-R | AGACGTCGACCGGGTTAATGATGATGATGATGATGGCTTTCTCCGTTAATTTCCTTATC | RcsB protein purification |