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. Author manuscript; available in PMC: 2015 Jul 29.
Published in final edited form as: J Biol Chem. 2001 Dec 14;277(8):6183–6187. doi: 10.1074/jbc.M110978200

Fig. 1. Response of the PFKFB3 gene to hypoxia, cobalt, and desferrioxamine.

Fig. 1

a, Hep-3B cells were maintained in normoxia (N), exposed to hypoxia (H), 0.5% O2, or incubated with 100 µm cobalt chloride (Co) or 130 µm desferrioxamine (D) for 6 h. Total RNA was extracted and analyzed for PFKFB3 and Glut-1 mRNA expression using RNase protection assays. b, Hep-3B cells where treated with desferroxamine, and total RNA was isolated at various times (Hrs) following the treatment and analyzed for Glut-1 (left) or PFKGFB3 (right) mRNA. c, responsiveness of the PFKFB3 gene to hypoxia (H) and desferrioxamine in wild-type (W.T.) and ρ° cells (25). In all figures, 18s represents 18 S ribosomal RNA.