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. Author manuscript; available in PMC: 2015 Jul 29.
Published in final edited form as: J Biol Chem. 2001 Dec 14;277(8):6183–6187. doi: 10.1074/jbc.M110978200

Fig. 4. Expression of the PFKFB3 gene in pVHL-deficient cells.

Fig. 4

a, total RNA from normoxic VHL deficient (−) renal carcinoma 786-0 cells and their VHL positive (+) controls where analyzed for PFKFB3, Glut-1, and VEGF mRNA expression by RNase protection assays. b, whole cell extracts from VHL (+) and VHL (−) were analyzed by Western blots using anti-HIF-2α and anti-VHL antibodies.