Effects of different factors on MEF2C mRNA expression in endothelial cells. HRECs were treated with VEGF, PlGF, FGF-2, and EGF, each at a concentration of 25 ng/mL, for 4 hours. Total RNA was isolated, and reverse transcription was performed with 1 μg RNA. Quantitative real-time PCR was used to detect the expression of MEF2C mRNA. Values were normalized to GAPDH gene expression. Results are representative of three independent experiments, each performed with duplicate samples. CN, control. *P < 0.05 versus control.