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. Author manuscript; available in PMC: 2015 Jul 29.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Apr 30;49(8):3640–3648. doi: 10.1167/iovs.08-1760

Figure 7.

Figure 7

Expression of a dominant-negative MEF2C mutant inhibits VEGF-stimulated endothelial cell migration. (A) A modified Boyden chamber assay25,26 was used to study VEGF stimulation of endothelial cell migration. HUVECs were transfected with a plasmid encoding dominant-negative MEF2C or corresponding control vector. Transfected cells were seeded into the upper chamber of 8-μm pore size transwells. The lower chamber contained medium with or without VEGF at 25 ng/mL. After incubation at 37°C for 4 hours, migrated cells were counted in eight different fields (20× objective). Data are presented as mean ± SD (B) Representative figures for modified Boyden chamber migration assay. *P < 0.05 versus control plasmid pcDNA 3.1 + VEGF. **P < 0.001 versus control plasmid without VEGF. Results shown are representative of five independent experiments.