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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: Nature. 2015 Mar 16;521(7553):545–549. doi: 10.1038/nature14247

Extended Data Figure 8. CryoEM single particle reconstruction of a subset of particles lacking the 14-stranded β barrel.

Extended Data Figure 8

a, Surface views of the cryoEM map lacking the β barrel. The map is unsharpened and has a resolution of 3.6 Å. b, Cross-section side view of superimposition of the unsharpened cryoEM map with the atomic model of the intact PA pore, showing the cryoEM map has the same conformation as the PA pore except for the absence of the 14-stranded β barrel. c, “Gold-standard” FSC (with an auto-mask that was corrected by phase randomization) between two independently refined maps. d, Top view of the Φ-clamp region of the cryoEM map (mesh) lacking the β barrel superimposed with the atomic model of the PA pore (ribbons), showing correct assembling of the Φ-clamp in the cryoEM map.