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. 2015 Jul 29;10(7):e0131842. doi: 10.1371/journal.pone.0131842

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 1 — MM2BH breast cancer cells, previously transfected to overexpress HER2, were transduced with lentiviral shRNAs (sR), scrambled or specific to αv-integrin, to create stable knockdown cell lines in combination with high or low HER2 protein. (A) Immunoblotting of protein expression in MM2BH lines with αv integrin knockdown in combination with high or low HER2 expression. (B) Relative expression of αv integrin and HER2 in MM2BH clones quantified by densitometry of immunoblots from three total cell lysates prepared at different culture passages. The band intensity was normalized to β-actin as a loading control. Error bars show SEM and * indicates values P<0.05 in comparison to control cells.