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. 2015 Jul 29;10(7):e0132804. doi: 10.1371/journal.pone.0132804

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Fig 2 — Glutaraldehyde-fixed, plastic-embedded sections stained with toluidine blue at low (A-C) and high (D-F) magnification. Naïve C57BL/6J corneas (left column) showed normal epithelial cell and stromal morphologies. Corneas with mild damage (middle column) and severe damage (right column) showed deposition of a crystalline substance between the epithelial and stromal layers. There was a loss of cellularity of the basal and wing epithelial cells and flattening of the stromal lamellae compared to corneas of naïve mice. Scale bars: A-C, 50 μm; D-F, 10 μm.