Table 1. Summary of experiments.
| Cohort | Experiment Date | Strain | Age | Sex | Source a | Anesthesia b | Drops and Hydration c | Yohimbine | OCT Retinal Scan d | Temperature Transponder | Supplemental Heat e | Eyes with damage |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1/10/12 | C57BL/6J | 3 wks | F | JAX AX4 | K/X | G + D | OU | Heating Pad | 3/4 (75%) | ||
| 1/10/12 | C57BL/6J | 3 wks | F | JAX AX4 | K/X | S + D | OU | Heating Pad | 3/4 (75%) | |||
| 1/10/12 | C57BL/6J | 3 wks | F | JAX AX4 | K/X | H + D | OU | Heating Pad | 3/4 (75%) | |||
| 1/10/12 | C57BL/6J | 3 wks | F | JAX AX4 | K/X | B + D | OS | Heating Pad | 6/8 (75%) | |||
| 1/10/12 | C57BL/6J | 3 wks | F | JAX AX4 | K/X | B + D | OU | Heating Pad | 9/12 (75%) | |||
| 2 | 1/24/12 | C57BL/6J | 12 wks | F | JAX AX4 | K/X | B + D | OS | Heating Pad | 2/8 (25%) | ||
| 3 | 4/2/12 | C57BL/6J | 3 wks | F | JAX AX4 | K/X | None | Heating Pad-cont. | 0/4 (0%) | |||
| 4/2/12 | C57BL/6J | 3 wks | F | JAX AX4 | K/X | None | Yes | Heating Pad-cont. | 0/8 (0%) | |||
| 4/2/12 | C57BL/6J | 3 wks | F | JAX AX4 | I | None | Heating Pad-cont. | 0/8 (0%) | ||||
| 4 | 2/12/13 | C57BL/6J | 3 wks | F | JAX MP15 | K/X | B | OU | Heating Pad | 8/8 (100%) | ||
| 2/12/13 | C57BL/6J | 3 wks | F | JAX MP15 | K/X | B | Yes | OU | Heating Pad | 0/8 (0%) | ||
| 2/12/13 | C57BL/6J | 3 wks | F | JAX MP15 | I | B | OU | Heating Pad | 0/8 (0%) | |||
| 5 | 8/8/13 | C57BLKS/J | 3 wks | F | IOWA | K/X | B | Yes | Heating Pad | 2/4 (50%) | ||
| 8/8/13 | C57BLKS/J | 18 wks | F | IOWA | K/X | B | Yes | Heating Pad | 0/4 (0%) | |||
| 6 | 10/29/13 | SJL/J | 3 wks | F | IOWA | K/X | B | Heating Pad | 4/10 (40%) | |||
| 7 | 10/30/13 | C57BLKS/J | 3 wks | F | IOWA | K/X | B | Yes | Physitemp | 2/6 (33.3%) | ||
| 10/30/13 | C57BLKS/J | 3 wks | F | IOWA | K/X | B | Yes | Physitemp-cont. | 2/8 (25%) | |||
| 8 | 3/6/14 | C57BL/6J | 3 wks | M/F | IOWA | K/X | B | Heating Pad | 4/6 (66.7%) | |||
| 3/6/14 | C57BL/6J | 3 wks | M/F | IOWA | K/X | P-N | Heating Pad | 0/8 (0%) | ||||
| 9 | 3/13/14 | C57BL/6J | 3 wks | F | IOWA | K/X | B | Heating Pad | 1/4 (25%) | |||
| 3/13/14 | C57BL/6J | 3 wks | F | IOWA | K/X | P-AT | Heating Pad | 0/6 (0%) | ||||
| 10 | 4/7/14 | C57BL/6J | 3 wks | F | JAX RB07 | K/X | B | Heating Pad | 0/10 (0%) | |||
| 4/7/14 | C57BL/6J | 3 wks | F | JAX RB07 | K/X | P-AT | Heating Pad | 0/10 (0%) | ||||
| 11 | 6/10/14 | C57BL/6J | 3 wks | F | JAX RB07 | K/X | B | Heating Pad | 7/14 (50%) | |||
| 6/10/14 | C57BL/6J | 3 wks | F | JAX RB07 | K/X | P-N | Heating Pad | 2/12 (14.3%) | ||||
| 6/10/14 | C57BL/6J | 3 wks | F | JAX RB07 | K/X | P-AT | Heating Pad | 2/14 (16.7%) | ||||
| 12 | 6/10/14 | SJL/J | 3 wks | F | JAX RB04 | K/X | B | Heating Pad | 0/14 (0%) | |||
| 6/10/14 | SJL/J | 3 wks | F | JAX RB04 | K/X | P-N | Heating Pad | 0/12 (0%) | ||||
| 6/10/14 | SJL/J | 3 wks | F | JAX RB04 | K/X | P-AT | Heating Pad | 0/14 (0%) |
aJAX, received from The Jackson Laboratory, colony indicated, experiments initiated the day following receipt
bK/X, ketamine/xylazine; I, isoflurane
cG, Genteal; S, Systane; H, Hypotears; B, balanced salt solution; D, dilation drops; P-N, petrolatum with Neomycin antibiotic; P-AT, petrolatum-based artificial tears
dOU, both eyes; OS, left eye
econt., continuous supplemental heat