Fig 6. Effect of ERK inhibition on PMA-stimulated NRF2i differentiation.

(A) Protein levels of phosphorylated ERK1/2 and total ERK1/2 were determined by western blot. SCi and NRF2i cells were incubated with PMA (10 ng/mL) for 30 min. (B-C) ERK1/2 inhibitor PD98059 (PD, 30 nM) was added to the cells for 24 h. Transcript levels for IL-1β (B) and IL-6 (C) were determined using RT-PCR analysis. Data represent the means ± SD of 3 experiments. ^aP < 0.05 compared with PMA-treated SCi. ^bP < 0.05 compared with PMA-treated NRF2i