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. 2015 Jul 29;10(7):e0134543. doi: 10.1371/journal.pone.0134543

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© 2015 Han et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — MCAO rats with or without HUK treatment were sacrificed and mRNA was extracted for the detection of apelin (A), APJ (B), VEGF (C) levels by real-time PCR. N = 6 per group. #p<0.05 versus sham group; ##p<0.01 versus sham group; *p<0.05 versus control group; **p<0.01 versus control group. Protein level of apelin in rat brain was determined by ELISA (F), while protein level of VEGF in rat brain was measured by Western blot (D). Quantitative data of VEGF blots at indicated time points (F). #p<0.05 versus sham group; *p<0.05 versus vehicle group, N = 6 repeats.