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. 2015 Jul 29;10(7):e0134543. doi: 10.1371/journal.pone.0134543

Fig 4. HUK up-regulated pro-angiogeic factors in ERK1/2 dependent way in cultured endothelial cells.

Fig 4

mRNA from cultured HAECs treated with HUK only or HUK plus U0126 was isolated for the measurement of VEGF (A), apelin (B), and APJ (C). N = 6 per group. (D) Western blot showed the activation of ERK pathway and VEGF expression in cultured HAECs incubated with HUK only or U0126 plus HUK for 15 min, 1 h, 3 h and 24 h. Statistic bar graph of p-ERK/ERK blots (E) and VEGF blots (F), N = 6 per group. (G) ELISA was used to measure apelin concentration in cultured cell medium. (H) F13A decreased VEGF expression up- regulated by HUK. (I) Quantitative data of VEGF blots intensity with or without F13A. #p<0.05 versus control group *p<0.05 versus HUK group; **p<0.01 versus HUK group; ##p<0.01 versus control group. N = 6 per group.