Fig 5. The levels of EcR and USP on polytene chromosome correlate with the expression of EcR target genes in salivary glands.

(A–C) Immunostaining of EcR (A, B, and C) and USP (A’, B’, and C’) in w ¹¹¹⁸ (control), cdk8 ^K185, and cycC ^Y5 polytene chromosomes, which were also stained with DAPI (A”, B”, and C”). Scale bar in (C”): 20μm. (D) Quantification of EcR target gene expression in salivary glands by qRT-PCR. (E) ChIP assay of USP binding to EcR target gene promoters in the w ¹¹¹⁸, cdk8 ^K185, and cycC ^Y5 mutant larvae. Ctrl: control; GPNS: guinea pig normal serum; IP: immunoprecipitation. * p < 0.05; ** p < 0.01 based on t-tests. Underlying numerical data and statistical analysis for Fig 5D and 5E can be found in S1 Data.