Fig 2. Recognition of predicted epitope peptides by rabbit anti-FliCBp sera as detected by Indirect ELISA.
Controls (A) composed of human serum albumin (HSA), crude B. pseudomallei, recombinant FliCBp, without primary antibody (No 10 Ab) and without secondary antibody (No 20 Ab) and F51-69, F96-111, F270-288 and F213-231 peptides (B) were coated at various concentration (1, 3 and 10 μg/ml) onto a 96-well polystyrene plate and probed with diluted rabbit antibodies to FliCBp and quantified by indirect ELISA. Results are represented by the Absorbance index (O.D.test−O.D.uncoated / O.D.uncoated). Experiments were performed in duplicate and results represent the mean of the Absorbance index ± SE.