Fig 3. Farnesol-specific tower formation in S. mutans static biofilm.

S. mutans UA159 static biofilms were grown in BM media containing 0.25% sucrose and 0.25% glucose, either with 2% P123 alone (A) or in the presence of 2% P123 formulated with farnesol (B) or triclosan (C). After 48 hours of growth, wells containing adherent biofilm were stained with LIVE/DEAD stain, and biofilm z-stacks were acquired at 400× magnification by CLSM. Representative orthogonal images of each biofilm are shown, representing 8–12 random fields of view. Scale bars = 20 μm.