Fig 6. Effect of P85-formulated farnesol on S. mutans sucrose-independent biofilm architecture.

UA159 static biofilms were grown in BM lacking sucrose (supplemented with 0.5% glucose) in the presence of P85 (A), P85F (B), F alone (C), or untreated (D). After 48 hours of growth, adherent biofilm was stained with LIVE/DEAD stain. Biofilm z-stacks were acquired at 400× magnification by CLSM, and orthogonal images are representative of 12 random fields of view acquired from 2 independent experiments. Scale bars = 20 μm.