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. Author manuscript; available in PMC: 2016 Jul 27.
Published in final edited form as: Dev Cell. 2015 Jul 2;34(2):192–205. doi: 10.1016/j.devcel.2015.05.003

Figure 6. Pathways regulating GSC cytokinesis are specific to the stem cell population.

Figure 6

Time lapse imaging of differentiating germ cell cysts; ABD-moeGFP and Myo-mCherry. Each panel is a 2–5 Z plane projection. Yellow dots=cells of two-cell cyst. White arrowhead=“original” IC bridge. Blue dots=daughter cells formed during mitosis. Blue arrowheads=“new” IC bridges. m=min. h=hour. *=Hub. Scale bar=5 microns.

(A1–A12)Division of a two-cell cyst. (A1–A4) The IC bridge of a two-cell cyst contained an F-actin ring for an extended period. (A5) F-actin at the IC bridge was only disassembled as the two cells rounded and entered mitosis. (A6) Myosin was enriched at the cleavage furrow during anaphase (arrows) between nascent daughter cells and a Myo-mCherry ring continued to mark the original IC bridge (arrowhead). (A7–A12) Contractile ring F-act is retained at the newly formed IC bridges of the four-cell cyst.

(B1–B6) Loss of LimK function has no effect on the F-actin ring at the IC bridge connecting two-cell cysts.

(C1–C6) Loss of ROK function has no effect on the F-actin ring at the IC bridge connecting two-cell cysts.

(D1–D6) As aurb1689 is a hypomorphic allele, abscission is not disrupted in all GSC-Gb pairs and some individual Gbs are released. Loss of AurB activity does not disrupt formation or maintenance of the IC bridge connecting two-cell cysts.