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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Stroke. 2015 Jul 9;46(8):2293–2298. doi: 10.1161/STROKEAHA.115.009876

Figure 3. Sirt1 and its targets are differentially regulated 14 days following a single application of RPC.

Figure 3

A, cortical Sirt1 protein was increased 14 days following a single application of RPC. Representative bands for Sirt1 are shown below the quantification graph. ⸗ = non-adjacent bands from the same blot. n=10; ** = p<0.01 compared to Veh. B, ChIP-qPCR analysis revealed Sirt1 binding to BDNF and UCP2 promoter regions was enhanced 14 days following a single application of RPC. n=3; * = p<0.05; ** = p<0.01. C, cortical BDNF levels were increased 14 days following a single application of RPC. Values are expressed as pg/mg of protein. n=7; * = p<0.05. D, synaptic UCP2 protein levels were decreased 14 days following a single application of RPC. Values were normalized to the mitochondrial marker voltage VDAC. The functional UCP2 dimer (70 kDa) was used for quantification, no 35 kDa monomer band was detected. Representative band are shown below the quantification. n=6; * = p<0.05.