Fig. 2.

ECs treated with IL-4 followed by complement that are resistant to killing rapidly regain efficient control of membrane permeability. ECs were incubated with 10 ng/ml IL-4 or medium alone for 48 h, washed, and incubated with 7.5% human serum for the indicated times. Then % LDH release (A) and % killing using neutral red (B) were measured. Results are means ± S.E.M. of 3 independent experiments that were performed in triplicate. In (A) LDH release, IL-4 vs. medium p > 0.05 for all time points except at 60 min p < 0.05. In (B) % killing, IL4 vs. medium p < 0.01 for all time points except at 15 min and 30 min p < 0.05.