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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Mol Biol. 2016;1353:13–23. doi: 10.1007/7651_2014_179

Fig. 1.

Fig. 1

Design of targeting vectors. ZFNs specific to the AAVS1 locus will cut the DNA in intron 1 of AAVS1. Integration of both targeting vectors will confer dual drug resistance to ESCs/iPSCs. The location of PCR screening primers and Southern blot probe is indicated