Fig. 3.

GFP expression in hematopoietic progenitors differentiated from hESC line with doxycycline-inducible GFP expression under different conditions. (a) GFP expression in hematopoietic progenitors treated with doxycycline for different lengths of time. Untreated hematopoietic progenitors (red) do not express GFP. Treatment with doxycycline for 2 days during differentiation is sufficient to induce GFP expression in hematopoietic progenitors (blue). When doxycycline was given for 8 days, the induction of GFP expression is stronger (black), suggesting that transgene expression does not get silenced when hESCs undergo hematopoietic differentiation. (b) The level of GFP expression in hematopoietic progenitors increases with the concentration of doxycycline added