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| Reagent/vector | Well #1 | Well #2 | Well #3 |
|---|---|---|---|
| AAVS1-ZFN-Left | 0.2 μg | 0.2 μg | 0.2 μg |
| AAVS1-ZFN-Right | 0.2 μg | 0.2 μg | 0.2 μg |
| AAVS1-SA-2A-PURO-TRE-GFP | 1.5 μg | 1 μg | 3 μg |
| AAVS1-SA-2A-NEO-CAG-RTTA | 1.5 μg | 3 μg | 1 μg |
| IMDM | Top up to 100 μl total volume | Top up to 100 μl total volume | Top up to 100 μl total volume |
| XtremeGENE 9 | 10.2 μl | 13.2 μl | 13.2 μl |
| TRE-GFP:CAG-RTTA ratio | 1:1 | 1:3 | 3:1 |
| Total DNA | 3.4 μg | 4.4 μg | 4.4 μg |
