Table 4.
Coefficient of correlation and efficiency of real-time PCR Standard curves obtained from serial diluted DNA extracts from maize flour (CRMs)
| Target | Mean and SD* | |||
|---|---|---|---|---|
| Extraction method | Coefficient of correlation (R2) | Angular coefficent (slope) | Efficiency** | |
| Plant DNA | Foodproof | 0.985 ± 0.002 | −3.197 ± 0.71 | 105.6 ± 3.3 |
| Genespin | 0.991 ± 0.006 | −3.303 ± 0.82 | 100.9 ± 3.5 | |
| Wizard | 0.994 ± 0.003 | −3.446 ± 0.10 | 96.6 ± 2.0 | |
| Dneasy | 0.995 ± 0.006 | −3.381 ± 0.45 | 97.6 ± 1.6 | |
| CTAB | 0.996 ± 0.005 | −3.303 ± 0.61 | 100.8 ± 2.7 | |
*SD standard deviation
**E, Efficiency, E = ([10(-1/slope)]-1) x 100%