FIGURE 4.

Normalized fold expression levels of selected miRNA/target mRNA nodes in fertilized roots and in SNF nodules of common bean plants under Alt for long period. High Al was added to (12 dpi) R. tropici-inoculated plants with active young nodules or to (12 days) fertilized plants for 7 days before harvesting. miRNAs and target genes expression levels were determined by qRT-PCR in nodules of inoculated plants or in roots of fertilized plants. Values represent the normalized expression ratios (stress:control) given as the average of three biological replicates. For ratios lower than 1, the inverse of the ratio was estimated and the sign was changed. Columns marked with star (^∗) represent significantly different means according to the statistical analysis (p ≤ 0.05). NAC1, NAM/ATAF/CUC transcription factor; SCL, SCARECROW-like protein; TIR1, TRANSPORT INHIBITOR RESPONSE 1-like protein; GRL1, GROWTH-REGULATING FACTOR 1; SP1L, SPIRAL-like protein 1; SPL, SQUAMOSA PROMOTER-BINDING protein-like; NF-YA1, NUCLEAR FACTOR YA1; CSD1, Cu/Zn SUPEROXIDE DISMUTASE.