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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Int J Cancer. 2014 May 9;135(11):2569–2578. doi: 10.1002/ijc.28908

Fig. 3. TLR2 signalling triggers glioma associated microglial MMP-9 up-regulation.

Fig. 3

(A) Microglia from WT and MyD88 or TLR 1, 2, 4, 6, 7 and 9 deficient mice were stimulated with GCM for 24h, cell supernatant was collected for gelatin zymography. Recombinant MMP-9 was used as a standard, GCM and DMEM were loaded as controls. Images were representative from 3 independent experiments. (B) Elisa was performed on cell supernatant for quantification of total MMP9 release. GCM and conditioned medium from primary cultured astrocytes were used as controls. Bars represent the mean±s.e.m. from 3 independent experiments. (C) Cell lysate and supernatant from WT and TLR2 KO microglia treated with GCM for 24h were further analyzed by western blot and compared to an untreated control (β-actin served as a loading control).