FIG 5.

Quantitation of chimV4-specific antibody-secreting cells isolated from bone marrow with an enzyme-linked immunospot assay (ELISPOT) (A) and chimV4-specific antibodies in middle ear fluid samples by quantitative slot blot analysis (B) after TCI with a band-aid vaccine placed in the postauricular region. TCI induced the production of immunogen-specific ASCs and antibodies, primarily of the IgM and IgG isotypes. The horizontal line in panel B indicates the limit of detection. Means ± SEMs are shown. **, P ≤ 0.01; ***, P ≤ 0.001, one-way ANOVA.