FIG 2.

Illustration of the lateral flow biplex test strips. (A) Photographs of individual patient sera showing a negative, Ov16 single positive, Wb123 single positive, and double positive result using the biplex format of the lateral flow strips. The position of the antigen lines is shown, and strips have been aligned by the control lines. (B) Comparison of Ov16 results demonstrating the similarity between the single format and biplex format lateral flow strips. The reactivity of 3 O. volvulus-infected patients with strong, average, and weaker IgG4 reactivity to Ov16 is shown in the single format and biplex format strips; in each set of strips, the Ov16 lines are aligned. The patients showed no reactivity to the Wb123 test line, the position of which is indicated by the labeled arrow in the top biplex strips. Photographs in panels A and B have had equivalent adjustments to approximate the color tone of the original strips.