TABLE 1.
Binding of the anti-O-PS MAbs in ELISA to Brucella strains and synthetic PSsa
| Antigen | Agglutination with monospecific serum: |
Binding titer (max. abs.) by ELISA of the following MAbb: |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| A | M | 2E11 (M) | 12G12 (C [A=M]) | 07F09 (C [A=M]) | 12B12 (C [M>A]) | 18H08 (C/Y [A=M]) | 04F9 (C/Y [A>M]) | 05D4 (C/Y [A>M]) | |
| Killed cells | |||||||||
| B. melitensis 16M (bv. 1) | − | + | 90 (2.506) | 7,290 (2.701) | 2,430 (2.791) | 90 (2.907) | 90 (2.858) | 30 (2.420) | 810 (2.432) |
| B. suis 1330 (bv. 1) | + | − | <10 | 7,290 (5.272) | 2,430 (4.573) | 30 (3.234) | 270 (4.368) | 7,290 (4.669) | 21,870 (4.317) |
| B. suis Thomsen (bv. 2) | + | − | <10 | 10 (2.422) | 10 (1.249) | <10 | 90 (3.356) | 7,290 (3.109) | 7,290 (3.211) |
| Synthetic PSsc (structural feature) | |||||||||
| NVG-125 (Di, α-1,3) | <10 | <10 | <10 | <10 | <10 | <10 | <10 | ||
| NVG-129 (Tri, α-1,3:1,2) | <10 | <10 | <10 | <10 | <10 | <10 | <10 | ||
| NVG-133 (Tri, α-1,2:1,3) | <10 | <10 | <10 | <10 | <10 | <10 | <10 | ||
| NVG-137 (Tetra, α-1,2:1,3:1,2) | <10 | <10 | <10 | <10 | <10 | <10 | <10 | ||
| JG5 (Penta, α-1,2:1,3:1,2:1,2) | <10 | 810 (4.278) | 270 (3.967) | <10 | 10 (1.486) | 10 (1.451) | 30 (1.859) | ||
| NVG-224 (Hexa, α-1,2:1,3:1,2:1,2:1,2) | <10 | 810 (4.559) | 270 (4.337) | <10 | <10 | 270 (3.405) | 90 (1.875) | ||
| NVG-220 [Hexa, α-(1,2)6] | <10 | <10 | <10 | <10 | 10 (1.661) | 2,430 (5.058) | 7,290 (5.062) | ||
| JG9 [Nona, α-(1,2)4:1,3:(1,2)3] | <10 | 810 (4.575) | 270 (3.724) | <10 | 10 (1.518) | 2,430 (4.927) | 7,290 (5.100) | ||
a
PSs, polysaccharides.
b
Results are expressed as titers of the MAbs, i.e., the highest dilutions of the MAbs giving an absorbance value above 1.0. Maximal absorbance (max. abs.) was observed mostly at a 1/10 dilution of the MAb.
c
Values inside the parentheses are the size and linkages of the PS structure.