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. 2015 Jul 30;5:12259. doi: 10.1038/srep12259

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Copyright © 2015, Macmillan Publishers Limited

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Testes from mice immunized with TH and adjuvant and injected with saline solution (vehicle) or recombinant Gal-1 (rGal-1) were removed, paraffin-embedded, and processed by in situ TUNEL assay. A- Representative microphotographs of TUNEL assay performed in EAO testis sections from mice treated with vehicle (A and C) or rGal-1 (B y D) and counterstained with eosin. TUNEL-positive cells are stained in blue. B- Quantitative assessment of TUNEL-positive cell number in 100 tubules per testis evaluated in a blinded fashion in each group. Each symbol represents a single mouse. (p = 0.05).