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. 2015 Jul 29;14(8):775–782. doi: 10.1128/EC.00066-15

FIG 2.

FIG 2

Role of vesicular fusion in swelling-induced ATP release. (A) Representative LS experiment showing cell size changes in suspensions of wild-type Dictyostelium amoebas immediately after exposure to hypotonic challenge. The cells were pretreated with NEM (1 mM; 15 min) or not (control). (B) Representative plot showing extracellular ATP concentrations with time. (C) Mean peak extracellular ATP concentrations measured under hypotonic conditions with (NEM) and without (control) NEM pretreatment for wild-type cells (n = 6). (D and E) Representative LS experiments showing cell size changes in suspensions of wild-type Dictyostelium amoebas and temperature-sensitive NSF mutant cells. (F) Mean peak extracellular ATP concentrations measured under hypotonic conditions for wild-type and NSF mutant amoebas (n = 6). *, P < 0.05. The error bars indicate standard errors of the means.