FIG 1.

Effects of acetylcholine on Candida albicans biofilm formation and metabolic activity in vitro. (A) Crystal violet assessment of C. albicans biomass after 24 h of growth in RPMI 1640 containing different concentrations of ACh (0 to 50 mg/liter). (B, C, and D) Graphical representations of phenotypic microarray analysis of C. albicans respiration during culture in RPMI and YNB for 24 h (B), 48 h (C), and 72 h (D) in the presence of different concentrations of ACh (0 to 50 mg/liter). All data are derived from triplicates of each condition performed in 3 independent experiments (n = 3). *, P < 0.05; **, P < 0.01; and ***, P < 0.001.