FIG 5.

Effects of acetylcholine on Candida albicans-induced hemocyte recruitment into Galleria mellonella tissues. Histological analysis of hemocyte recruitment into larval tissues was performed using PAS staining 24, 48, and 72 h postinoculation. The control groups are larvae inoculated with PBS (A, E, and I) or ACh (B, F, and J) alone. Larvae inoculated with C. albicans alone (C, G, and K) and C. albicans plus ACh (D, H, and L) are also represented. Extensive hemocyte recruitment is highlighted with black arrows. Hemocyte nodule formation is highlighted with a black asterisk. Representative images are shown from histological analysis of >3 larvae for each condition from 3 independent experiments. Fb, fat body; GI, gastrointestinal tract; T, trachea. Scale bars, 100 μm.