FIG 6.

CTH4 is not required for formation and accumulation of docked mucocysts. (A) (Top) docked mucocysts in fixed wild-type cells, immunolabeled using MAb 4D11, which recognizes Grt1p (left two images), and MAb 5E9, which recognizes Grl3p (right two images). Shown are optical surface and cross sections. (Bottom) parallel immunostaining of Δcth4 cells shows patterns indistinguishable from the wild type. Scale bars = 10 μm. (B) Electron micrographs of mucocysts (labeled with asterisks) in wild-type and Δcth4 cells. The mucocyst cores in both wild-type and Δcth4 cells are organized as visible lattices. Scale bars = 0.2 μM.