TABLE 1.
Conidial surface proteins identified in the wild-type and ΔsitA mutant strains
| A. fumigatus protein | Function | Mass (kDa) | Length (aaa) | Ratio (ΔsitA/WT)b | % coveragec |
|
|---|---|---|---|---|---|---|
| Mutant | WT | |||||
| Conidial surface proteins absent in the ΔsitA mutant strain | ||||||
| Afu1g17410 | Beta-glucosidase | 84 | 782 | Only in WT | NI | 2 |
| Afu3g14030 | Alkaline phosphatase, phoB regulated | 72 | 653 | Only in WT | NI | 17 |
| Afu2g00690 | Glucan 1,4-alpha-glucosidase activity; role in polysaccharide metabolic process and Golgi apparatus, endoplasmic reticulum, and prospore membrane localization | 67 | 631 | Only in WT | NI | 6 |
| Afu2g17530 | Laccase involved in conidial pigment biosynthesis | 65 | 587 | Only in WT | NI | 15 |
| Afu7g05140 | Putative class III family 18 chitinase | 46 | 448 | Only in WT | NI | 16 |
| Afu3g00270 | Cell wall glucanase; predicted glycophosphatidylinositol (GPI) anchor; secreted protein | 44 | 450 | Only in WT | NI | 5 |
| Afu2g05240 | Orthologues have extracellular region localization | 42 | 400 | Only in WT | NI | 11 |
| Afu2g00680 | Open reading frame (ORF), uncharacterized; has domain(s) with predicted catalytic activity | 40 | 375 | Only in WT | NI | 8 |
| Afu7g05650 | ORF, uncharacterized; orthologue of A. fumigatus A1163 (AFUB_091230) | 23 | 225 | Only in WT | NI | 8 |
| Afu5g01420 | ORF, uncharacterized; orthologue(s) have extracellular region localization | 22 | 205 | Only in WT | NI | 30 |
| Afu2g13600 | Pyruvate dehydrogenase (acetyl-transferring) kinase activity; role in carbon utilization, peptidyl-serine phosphorylation, and mitochondrion localization | 20 | 187 | Only in WT | NI | 8 |
| Conidial surface proteins absent in the WT strain | ||||||
| Afu8g05020 | NagA, secreted N-acetylhexosaminidase; highly expressed in biofilm | 67 | 600 | Only in ΔsitA | 8 | NI |
| Afu2g02100 | Dihydrolipoamide dehydrogenase; reacts with rabbit immunosera exposed to A. fumigatus conidia | 54 | 513 | Only in ΔsitA | 27 | NI |
| Afu2g01170 | Gel1, 1,3-beta-glucanosyltransferase with a role in elongation of 1,3-beta-glucan chains; GPI-anchored protein; constitutively expressed during hyphal growth; hypoxia-induced protein | 48 | 452 | Only in ΔsitA | 6 | NI |
| Afu7g05740 | NAD-dependent malate dehydrogenase; protein abundant in conidia; immunoreactive; reacts with rabbit immunosera exposed to A. fumigatus conidia; induced by l-tyrosine; transcript downregulated in response to hypoxia | 35 | 340 | Only in ΔsitA | 17 | NI |
| Afu6g10930 | ORF, uncharacterized; protein of unknown function | 30 | 290 | Only in ΔsitA | 8 | NI |
| Afu6g14370 | AraC-like ligand binding domain protein | 23 | 213 | Only in ΔsitA | 9 | NI |
| Afu3g00880 | Putative adhesin protein; MedA-regulated transcript; transcript repressed by exposure to human airway epithelial cells | 21 | 219 | Only in ΔsitA | 13 | NI |
| Afu3g09390 | Laminin-binding protein with extracellular thaumatin domain; predicted adhesin-like protein; expression induced in biofilm; repressed by exposure to artemisinin | 18 | 177 | Only in ΔsitA | 14 | NI |
| Afu1g01980 | ORF, uncharacterized; orthologue(s) have role in hyphal growth, response to cold, response to heat, response to oxidative stress, response to salt stress, sporocarp development involved in sexual reproduction | 18 | 179 | Only in ΔsitA | 6 | NI |
| Afu5g01990 | ORF, uncharacterized; BYS1 domain protein | 16 | 156 | Only in ΔsitA | 12 | NI |
| Afu4g09320 | DpplV, extracellular dipeptidyl-peptidase; predicted signal sequence for secretion; induced by growth on BSA as a sole nitrogen source; expression dependent on PrtT | 85 | 765 | 30.5 | 18 | NI |
| Conidial surface proteins more highly expressed in the ΔsitA mutant strain | ||||||
| Afu5g10490 | Amidase; secreted protein; fibrinogen binding | 65 | 611 | 18.8 | 5 | 3 |
| Afu5g03540 | Orthologues have flavin-linked sulfhydryl oxidase activity and roles in oxidation-reduction process and extracellular region localization | 42 | 386 | 11.7 | 12 | 14 |
| Afu4g13750 | Mep20, penicillolysin/deuterolysin metalloprotease; predicted signal sequence for secretion | 39 | 370 | 9.6 | 5 | 5 |
| Afu1g06390 | Tef1, translation elongation factor EF-1 alpha subunit; protein abundant in conidia; protein induced by heat shock | 53 | 494 | 4.9 | 14 | 3 |
| Afu3g08290 | Aspartyl aminopeptidase; immunoreactive; secreted protein; induced by growth on BSA as a sole nitrogen source | 54 | 504 | 3.9 | 6 | 5 |
| Afu8g00710 | ORF, uncharacterized; has domain(s) with predicted role in defense response, negative regulation of growth | 10 | 101 | 3.2 | 34 | 48 |
| Afu2g09030 | DppV, secreted dipeptidyl-peptidase; induced by growth on BSA as a sole nitrogen source; immunoreactive protein | 79 | 721 | 2.9 | 24 | 12 |
| Afu7g07010 | Adh1, alcohol dehydrogenase; predicted gene pair with AFUA_5G06240 (alcC); induced by l-tyrosine; transcript upregulated in conidia exposed to neutrophils | 37 | 353 | 2.0 | 5 | 4 |
| Afu5g09240 | Sod1, Cu/Zn superoxide dismutase; recognized by sera from aspergillosis patients; highly expressed in conidia; transcript induced by copper starvation, menadione, gliotoxin, and growth at high temp; protein induced by hydrogen peroxide | 15 | 154 | 2.0 | 28 | 30 |
| Afu3g02270 | Cat1, mycelial catalase; induced in hyphae exposed to neutrophils; protein induced in amphotericin B and H2O2; stuA-dependent upregulation in developmentally competent hyphae; hypoxia repressed; SrbA regulated; repressed by gliotoxin exposure | 79 | 728 | 2.0 | 22 | 21 |
| Afu5g01120 | ORF, uncharacterized; orthologue of Aspergillus nidulans FGSC A4 (AN8927), Aspergillus oryzae RIB40 (AO090003001298), Aspergillus flavus NRRL 3357 (AFL2T_01773), and Neosartorya fischeri NRRL 181 (NFIA_041050) | 36 | 354 | 1.8 | 4 | 9 |
| Afu2g12630 | Aspf13, allergen Asp f 13; putative alkaline serine protease; higher expression in biofilm than in planktonic cells; transcript induced by growth on hydrogen peroxide | 15 | 152 | 1.8 | 43 | 44 |
| Afu3g03060 | Aspf34, allergen Asp f 34; putative PhiA family cell wall protein; induced by calcium; repressed by exposure to artemisinin | 19 | 185 | 1.8 | 9 | 9 |
| Afu1g12070 | ORF, uncharacterized; orthologues have glycine dehydrogenase (decarboxylating) activity, roles in glycine catabolic process, one-carbon metabolic process, protein lipoylation, and mitochondrion localization | 18 | 175 | 1.6 | 9 | 9 |
| Afu3g15090 | Adenosine deaminase family protein; secreted protein; predicted secretory signal sequence; orthologue of A. nidulans AN2494 | 66 | 587 | 1.3 | 16 | 15 |
| Conidial surface proteins more highly expressed in the WT | ||||||
| Afu1g15730 | 40S ribosomal protein S22 | 14 | 130 | 0.9 | 11 | 11 |
| Afu1g14450 | ExgO, exo-beta-1,3-glucanase; secreted protein; predicted secretory signal sequence; orthologue of A. nidulans AN0779 | 10 | 947 | 0.8 | 10 | 13 |
| Afu3g07030 | Gta1, glutaminase A; secreted protein; predicted secretory signal sequence | 76 | 691 | 0.8 | 16 | 20 |
| Afu3g00590 | AspHS, Asp-hemolysin; hemolytic toxin; highly secreted; enriched in conidia; expression increases in vivo; binds lysophosphatidylcholine | 15 | 139 | 0.7 | 20 | 25 |
| Afu1g05770 | Exg12, secreted beta-glucosidase; predicted secretory signal sequence; fibrinogen binding | 94 | 873 | 0.6 | 10 | 20 |
| Afu1g10970 | Alpha-1,2-mannosidase family protein | 92 | 839 | 0.6 | 2 | 19 |
| Afu6g14470 | Orthologue of A. nidulans FGSC A4 (AN5353), Aspergillus niger CBS 513.88 (An02g05680), A. oryzae RIB40 (AO090103000108), Aspergillus wentii (Aspwe1_0131073), and Aspergillus sydowii (Aspsy1_0050268) | 26 | 234 | 0.5 | 10 | 18 |
| Afu2g00967 | Orthologue of A. nidulans FGSC A4 (AN7635/binA and AN11904), A. fumigatus Af293 (Afu3g02216 and Afu7g00610), and A. niger CBS 513.88 (An10g00560) | 21 | 196 | 0.5 | 7 | 8 |
| Afu6g03210 | ConJ, protein of unknown function; conidium-enriched protein; orthologue of Neurospora crassa | 87 | 83 | 0.4 | 35 | 26 |
| Afu8g00630 | Uncharacterized; Orthologues have extracellular region localization | 37 | 347 | 0.3 | 27 | 41 |
| Afu4g09280 | ORF, uncharacterized; orthologue of A. niger CBS 513.88 (An02g00330), Neosartorya fischeri NRRL 181 (NFIA_106720), A. niger ATCC 1015 (36613-mRNA), and Aspergillus carbonarius ITEM 5010 (Acar5010_203109) | 18 | 167 | 0.2 | 28 | 54 |
| Afu4g11800 | Alp1, secreted alkaline serine protease; cleaves human complement components C3, C4, and C5; predicted signal sequence for secretion; fibrinogen binding | 42 | 403 | 0.2 | 12 | 22 |
| Afu6g12070 | FmqD, flavoprotein amide oxidase (FAD); has FAD activity; member of the fumiquinazoline-biosynthetic cluster; may contain an N-terminal signal sequence and multiple N-glycosylation sites; transcript downregulated in response to voriconazole | 54 | 497 | 0.2 | 5 | 14 |
| Afu1g14560 | MsdS, 1,2-alpha-mannosidase; secreted protein; fibrinogen binding | 53 | 493 | 0.2 | 2 | 7 |
| Afu7g06140 | Exg13, secreted 1,4-beta-d-glucan glucanhydrolase | 78 | 739 | 0.1 | 2 | 7 |
| Afu5g02330 | Aspf1, Aspf1 antigen/allergen; RNase mitogillin family of cytotoxins; similar to restrictocin; inhibits protein synthesis in mammalian cells; 18-kDa IgE-binding protein; expression increases in vivo; biofilm-induced vs planktonic cells | 19 | 176 | 0.05 | 7 | 17 |
a
aa, amino acids.
b
The values are related to the spectral counts of three repetitions for each strain. WT, wild-type strain.
c
NI, not identified.