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Relative aspecific integration is much lower in the lig4 mutants than in the wild-type strain. Relative aspecific integration values, calculated as the number of false-positive transformants of each batch/number of false-positive transformants of the wild-type strain of the batch, for deletion cassettes targeting ADE2, ARG8, or CNA1 are shown. P < 0.05 for comparison of the wild type to the lig4 mutants.
