TABLE 2.
Correct transformation efficiency in wild-type C. glabrata and two independent lig4 mutant strainsa
| Gene deletion tested and batch no.b | No. of correct gene deletions/total no. of transformants |
||
|---|---|---|---|
| Wild type | lig4 (a) | lig4 (b) | |
| ADE2 | |||
| Batch1 | 1/105 | 1/3 | 0/1 |
| Batch2 | 0/71 | 0/0 | 0/2 |
| Batch3 | 0/31 | 0/2 | 0/2 |
| Batch4 | 0/29 | 0/6 | 1/5 |
| Overall | 1/236 | 1/11 | 1/10 |
| ARG8 | |||
| Batch1 | 1/332 | 0/1 | 1/4 |
| Batch2 | 0/17 | 0/2 | 0/0 |
| Batch3 | 0/40 | 0/0 | 0/1 |
| Batch4 | 0/45 | 0/0 | 1/2 |
| Overall | 1/434 | 0/3 | 2/7 |
| CNA1 | |||
| Batch1 | 0/37 | 0/0 | 0/0 |
| Batch2 | 0/33 | 0/0 | 0/0 |
| Batch3 | 0/39 | 0/1 | 0/0 |
| Batch4 | 0/120 | 0/0 | 0/0 |
| Batch5 | 0/196 | 0/1 | 1/3 |
| Batch6 | 0/121 | 0/0 | 0/1 |
| Overall | 0/546 | 0/2 | 1/4 |
a
For each experiment, the wild-type strain and the two lig4 mutants were incubated in 50 ml YPD medium at an OD600 of 0.2 and grown at 37°C to an OD600 of 1.5. Competent cells harvested from one such culture were defined as one batch.
b
Data are from at least four independent transformation rounds in the wild-type background and in the two independent lig4 mutants.