Figure 4.
The analysis of ERS molecule Grp78 by IHC Grp78 was mainly localized in the cytoplasm. Consistent with the results of the real-time PCR, the protein expression of Grp78 was examined, and the peaks of Grp78 emerged in the late-luteal stage on day 14; the levels decreased on day 20. a and e: early-luteal stage (2 d); b and f: mid-luteal stage (7 d); c and g: late-luteal stage (14 d); d and h: late-luteal stage (20 d). The picture in the upper right corner of h indicates the enlargement. Scale bar: a, b, c, and d: 50 μm; e, f, g, and h: 100 μm. ERS indicates endoplasmic reticulum; Grp78, glucose-regulated protein 78; IHC, immunohistochemistry; Grp78, glucose-regulated protein 78; PCR, polymerase chain reaction; d, day.