Table 1.
Primer Sequences Used for Real-Time Quantitative PCR.
| Target gene | GenBank Accession No. | Primer Sequence | Product Size, bp | Annealing Temperature, °C |
|---|---|---|---|---|
| XBP1s | NM_001271731.1 | F:5′-GGTCTGCTGAGTCCGCAGCAGG-3′ R:5′-GAAAGGGAGGCTGGTAAGGAAC-3′ |
232 | 60 |
| ATF6α | NM_001107196.1 | F: 5′-GGG AGT GAG CTG CAG GTG TA-3′ R: 5′-TTA TGG GTG GTA GCT GGT AA-3′ |
137 | 60 |
| eIF2α | NM_019356.1 | F:5′-GCTTGCTATGGTTACGAAGGC-3′ R:5′-CATCACATACCTGGGTGGAG-3′ |
120 | 60 |
| IRE1α | NM_001191926.1 | F: 5′-CGA GCC ATG AGGAAT AAG AG-3′ R: 5′-GGA AAC GTG ATG TGA AGT AG-3′ |
109 | 60 |
| Grp78 | NM_013083.2 | F:5′-AGAAACTCCGGCGTGAGGTAGA-3′ R:5′-TTTCTGGACAGGTTTCATGGTAG-3′ |
176 | 60 |
| CHOP | NM_024134.2 | F: 5′-AGCTGGAAGCCTGGTATGAGGA-3′ R: 5′-AGCTAGGGATGCAGGGTCAA-3′ |
134 | 60 |
| Caspase 12 | NM_130422.1 | F:5′-CTGGCCCTCATCATCTGCAACAA-3′ R: 5′-CGGCCAGCAAACTTCATTAAT-3′ |
173 | 60 |
| Caspase 3 | NM_012922.2 | F: 5′-ACTGGAAAGCCGAAACTCTTC-3′ R: 5′-AGTTCCACTGTCTGTCTCAATA-3′ |
81 | 60 |
| GAPDH | NM_017008.4 | F: 5′-GGCATCGTGGAAGGGCTC-3′ R: 5′-GACCTTGCCCACAGCCTT-3′ |
156 | 60 |
Abbreviations: PCR, polymerase chain reaction; XBP1, X-box binding protein 1; ATF6α, activating transcription factor 6α; eIF2α, eukaryotic initiation factor 2α; IRE1α, inositol-requiring protein 1α; Grp78, glucose-regulated protein 78; CHOP, CCAAT/enhancer-binding protein homologous protein.