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. 2015 Jul 30;5:12615. doi: 10.1038/srep12615

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Fluorescence microscope images of longitudinal spinal cord tissue sections in a control (intact) (a,c,e) and an SCI (injured) rat 8 weeks after complete transection injury (b,d,f). In the SCI rats, the images were taken 0.6 mm above and below injury. Sections were stained with Iba-1 (green). (a) and (b) were taken at 50x magnification and encompass an entire section through the middle of the spinal cord, near the central canal. (c–f) were taken at 200x magnification in the grey matter. Microglia in the intact spinal cord (c,e) have elaborate thin processes which extend in various directions (arrow heads). Note increased staining intensity, rounder cell bodies and shorter and thicker processes (arrows) rostral (d) and caudal (f) to the site of injury. Insets show microglia cell morphology at 400x magnification. Scale bar (a,b), 250 μm. Scale bar (c–f), 75 μm.