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Inactivation of the cj1387c, cj1388, and cj0327 genes does not decrease swarming motility. Brucella broth supplemented with 0.4% agar and 0.01% TTC were inoculated with 5 μl of overnight culture and incubated in microaerobic conditions at 37°C. Halo formation was measured after 24 and 48 h and expressed as a percentage of the wild-type. Results are the mean from at least three biological replicates. An asterisk denotes statistically significant results, based on a one-sample t-test.
