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. 2015 Jul 30;6:770. doi: 10.3389/fmicb.2015.00770

Table 1.

Bacterial strains described in this study.

Strain Descriptiona
E. coli STRAINS
Top10 General cloning strain
BL21 (DE3) Over-expression strain compatible with the pET expression system (T7 promoter)
BL21 (DE3)::pCASO40 Cj1388 expressed in pET28a resulting in an N-terminal his-tagged protein
BL21 (DE3)::pCASO53 Cj1388Cys71Ser expressed in pET28a resulting in an N-terminal his-tagged protein
C. jejuni STRAINS
NCTC 11168 Wild-type C. jejuni (Parkhill et al., 2000)
11168 Δcj1387c NCTC 11168 cj1387c::catR
11168 Δcj1387cKm NCTC 11168 cj1387c::kanR
11168 Δcj1388 NCTC 11168 cj1388::kanR
11168 Δcj0327 NCTC 11168 cj0327::catR
11168 Δcj1388cj0327 NCTC 11168 cj1388::kanR cj0327::catR
11168 ΔflaAB NCTC 11168 (cj1338-39c)::kanR (Reuter and van Vliet, 2013)
11168 ΔflaABcj1387c NCTC 11168 flaAB::kanR cj1387c::catR
11168 ΔpflA NCTC 11168 cj1565c::kanR
11168 ΔpflAcj1387c NCTC 11168 cj1565c::kanR cj1387c::catR
11168 Δcj1387c::cj1387cfdxApr* NCTC 11168 cj1387c::catR cj0046::cj1387cfdxApr*kanR
11168 Δcj1387c::cj1388fdxApr* NCTC 11168 cj1387c::catR cj0046::cj1388fdxApr*kanR
11168 Δcj0327::cj0327fdxApr* NCTC 11168 cj0327::catR cj0046::cj0327fdxApr*kanR
11168::cj1387cfdxApr* NCTC 11168 cj0046::cj1387cfdxApr*kanR
11168 Δcj1388::cj1388* NCTC 11168 cj1388::kanR cj0046::cj1388*catR
11168 Δcj1388::cj1388cys71ser* NCTC 11168 cj1388::kanR cj0046::cj1388cys71ser*catR
a

kanR, kanamycin antibiotic resistance cassette; catR, chloramphenicol antibiotic resistance cassette;

*

denotes complementation construct: fdxApr, gene under control of the constitutive fdxA promoter.