Table 1.
Strain | Descriptiona |
---|---|
E. coli STRAINS | |
Top10 | General cloning strain |
BL21 (DE3) | Over-expression strain compatible with the pET expression system (T7 promoter) |
BL21 (DE3)::pCASO40 | Cj1388 expressed in pET28a resulting in an N-terminal his-tagged protein |
BL21 (DE3)::pCASO53 | Cj1388Cys71Ser expressed in pET28a resulting in an N-terminal his-tagged protein |
C. jejuni STRAINS | |
NCTC 11168 | Wild-type C. jejuni (Parkhill et al., 2000) |
11168 Δcj1387c | NCTC 11168 cj1387c::catR |
11168 Δcj1387cKm | NCTC 11168 cj1387c::kanR |
11168 Δcj1388 | NCTC 11168 cj1388::kanR |
11168 Δcj0327 | NCTC 11168 cj0327::catR |
11168 Δcj1388cj0327 | NCTC 11168 cj1388::kanR cj0327::catR |
11168 ΔflaAB | NCTC 11168 (cj1338-39c)::kanR (Reuter and van Vliet, 2013) |
11168 ΔflaABcj1387c | NCTC 11168 flaAB::kanR cj1387c::catR |
11168 ΔpflA | NCTC 11168 cj1565c::kanR |
11168 ΔpflAcj1387c | NCTC 11168 cj1565c::kanR cj1387c::catR |
11168 Δcj1387c::cj1387cfdxApr* | NCTC 11168 cj1387c::catR cj0046::cj1387cfdxApr*kanR |
11168 Δcj1387c::cj1388fdxApr* | NCTC 11168 cj1387c::catR cj0046::cj1388fdxApr*kanR |
11168 Δcj0327::cj0327fdxApr* | NCTC 11168 cj0327::catR cj0046::cj0327fdxApr*kanR |
11168::cj1387cfdxApr* | NCTC 11168 cj0046::cj1387cfdxApr*kanR |
11168 Δcj1388::cj1388* | NCTC 11168 cj1388::kanR cj0046::cj1388*catR |
11168 Δcj1388::cj1388cys71ser* | NCTC 11168 cj1388::kanR cj0046::cj1388cys71ser*catR |
kanR, kanamycin antibiotic resistance cassette; catR, chloramphenicol antibiotic resistance cassette;
denotes complementation construct: fdxApr, gene under control of the constitutive fdxA promoter.