Table 2.
Plasmids described in this study.
| Plasmids | Descriptiona |
|---|---|
| pNEB193 | General cloning vector (NEB) |
| pET28a | Over-expression strain for making N-terminally his-tagged proteins |
| pCfdxA | Complementation plasmid containing cj0046 flanks, Chloramphenicol resistance cassette and fdxA promoter (Reuter and van Vliet, 2013) |
| pC46 | Complementation plasmid containing cj0046 flanks, Chloramphenicol resistance cassette (for native promoter complementation (Reuter and van Vliet, 2013) |
| pCj1387::KanC1 | cj1387c disruption plasmid, kanR |
| pCj1387::CatC1 | cj1387c disruption plasmid, catR |
| pCASO24 | cj1387c complementation plasmid—fdxA promoter, kanR |
| pCASO29 | cj1388 disruption plasmid, kanR |
| pCASO36 | cj0327 disruption plasmid, catR |
| pUCΔpflA | cj1565c (pflA) disruption plasmid, kanR |
| pCASO40 | cj1388 cloned into pET28a |
| pCASO41 | cj1388 complementation plasmid—fdxA promoter, catR |
| pCASO52 | cj1388cys71ser complementation plasmid—fdxA promoter, catR |
| pCASO53 | cj1388cys71ser cloned into pET28a |
| pCASO62 | cj0327 complementation plasmid—fdxA promoter, kanR |
| pMARKan9 | Source of Kanamycin resistance cassette (van Vliet et al., 1998) |
| pAV35 | Source of Chloramphenicol resistance cassette (van Vliet et al., 1998) |
a
kanR, kanamycin resistance; catR, chloramphenicol resistance.