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. 2015 Jul 30;5:12584. doi: 10.1038/srep12584

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Copyright © 2015, Macmillan Publishers Limited

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GPR103-CFP and OX1R-YFP were expressed individually or co-expressed in HEK293T cells: YFP (a) GPR103-CFP (b) GPR103-CFP and YFP (c) OX1R-YFP (d) GPR103-CFP and OX1R-YFP (e,f) Upon stimulation with 100 nM QRFP-43 (e) or OXA (f) there is significant change in FRET. GPR103-CFP and OX2R-YFP were expressed individually or co-expressed in HEK293T cells: YFP (g) GPR103-CFP (h) GPR103-CFP and YFP (i) OX2R-YFP (j) GPR103-CFP and OX2R-YFP (k–m) FRET was detectable upon stimulation with QRFP (k) OXA (l), OXB (m; all at 100 nM). Lowest FRET intensity is in purple/black and the highest in red.